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Thermo Fisher
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Miltenyi Biotec
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Image Search Results
Journal: Nutrients
Article Title: Vitamin D Insufficiency Exacerbates Adipose Tissue Macrophage Infiltration and Decreases AMPK/SIRT1 Activity in Obese Rats
doi: 10.3390/nu9040338
Figure Lengend Snippet: Vitamin D insufficiency increases macrophage infiltration in adipose tissue. ( A ) Macrophage marker, F4/80 immunohistochemistry of epididymal adipose tissue (scale bar, 50 μm; magnification, 40×); the black arrows indicate a CLS; ( B ) Number of CLS was quantified from multiple histologic sections and expressed as the mean ± SEM. ** p < 0.01 compared to NOR. ## p < 0.01 compared to HF. NOR, 10% fat diet with 1000 IU vitamin D ( n = 9); HF, 45% fat diet with 1000 IU vitamin D ( n = 7); HF+LVD, 45% fat diet containing 25 IU vitamin D ( n = 7).
Article Snippet: Histological sections of adipose tissue were dewaxed in xylenes, rehydrated and stained using
Techniques: Marker, Immunohistochemistry
Journal: Stem cell research
Article Title: Migration of CD11b + Accessory Cells During Murine Lung Regeneration
doi: 10.1016/j.scr.2012.12.006
Figure Lengend Snippet: Subpopulation kinetics of CD11b+ cells days after pneumonectomy. Multiparameter flow cytometry demonstrated the composition of the CD45+/CD11b+ cells in the interstitial tissue (A) and the airspace (B) after pneumonectomy. A) The interstitial compartment demonstrated a significant increase in neutrophils (CD11b+, Gr-1hi+ cells on day7 compared with day 0; *p<.04) and dendritic cells (CD11b+, CD11c+ cells on day7 compared with day 0; *p<.03), but the increase in monocytes (CD11b+, F4/80+ cells) was not significant (p<0.11). B) The airspace compartment demonstrated a significant increase in monocytes (CD11b+, F4/80+ cells on day14 compared with day0; *p<.02) and dendritic cells (CD11b+, CD11c+ cells on day14 compared with day 0; *p<.02), but there was no significant change in the percentage of neutrophils (CD11b+, Gr-1hi+ cells on day7 and 14 compared with day 0; p<.18).
Article Snippet: For flow cytometric lung cell analyses, fluorescein isothiocynate (FITC)-, phycoerythrin (PE)-, allophycocyanin (APC)-, PE-Cy7-conjugated monoclonal antibodies (mAb) were used: anti-CD45 mAb (FITC, rat IgG2b, clone 30-F11, eBioscience), anti-CD11c mAb (PE or APC; hamster IgG, clone N418, eBioscience), anti-CD11b mAb (PE-Cy7, rat IgG2b, clone M1/70, BD Bioscience), anti-Gr-1 mAb (APC, rat IgG2b, clone RB-8C5, eBioscience), anti-Ly6G mAb (biotin, clone 1A8, BD Bioscience),
Techniques: Flow Cytometry